233.Ammonium affects mitochondrial distribution and function in mouse 2-cell embryos

Mitochondrial distribution and function in oocytes and early embryos.

Active mitochondria relocate during oocyte maturation or fertilization in several species. Detailed studies with hamster oocytes and early embryos reveal a pattern of active mitochondria migrating to surround the pronuclei to form a pattern that persists through the early cleavage stages. Although the functional significance of this relocation is unknown, it appears to be an important part of n...

The Effect of Vitrification and in vitro Culture on the Adenosine Triphosphate Content and Mitochondrial Distribution of Mouse Pre-Implantation Embryos

Background: The mitochondria are an important source of adenosine triphosphate (ATP) production in pre-implantation embryo. Therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and ATP content. Methods: The embryos at 2-PN, 4-cell and blastocyst stages were collected from the oviduct of st...

Effects of Visible Light on the Development of Mouse 2-Cell Embryos

Introduction: Mammalian embryos as well as oocytes are prone to various doses of visible light during manipulations in laboratory. The present study was designed to investigate the effects of visible light on the development of mouse 2- cell embryos. Method: Non-pregnant female NMRI mice were super-ovulated with i.p. injection of 7.5 iu PMSG followed 48 hours later with 10 iu hCG. Forty eight h...

Cell distribution in chimaeric mouse embryos before implantation.

When two mouse embryos are aggregated at the 8-cell stage (Tarkowski, 1961) the resulting adult chimaera (Mintz, 1965) shows evidence of extensive mixing of the two cell populations. For example, the analysis of eye muscles each derived from a single somite showed that, in chimaeric mice, all of 33 such muscles contained cells from both components of the chimaera (Gearhart & Mintz, 1972). How t...

The Effects of Ethanol and Strontium on Growth and Development of Two-Cell Arrested Mouse Embryos